Proceedings of the 8th International Conference on Sustainable Innovation, Volume 4 Issue No. 1 (2025)
An Experimental Model of Cigarette Smoke-Induced Oxidative Stress in Rats
Rahmah1,2a),Harsono Salimo1,Brian Wasita1,Eti Poncorini Pamungkasari1, Yusup Subagio1, Risya Cilmiaty1, Soetrisno1
1Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta,Indonesia
2School of Nursing, Faculty of Medicine and Health Science, Universitas Muhammadiyah Yogyakarta, Yogyakarta, Indonesiaa)
Correspondingauthor:rahmah@umy.ac.id
Abstract.
Cigarette smoke is a primary cause of several respiratory diseases in children due to its high content of free radicals, acting as a gateway for various bacteria and viruses in children’s respiratory systems, leading to oxidative stress in the pulmonary. Malondialdehyde expression in the lung exhibits oxidative stress processes. Treatment development in children’s lungs needs appropriate animal models. However, most animal models utilize adult rats with cigarette exposure. This research aims to develop a rat model with lung oxidative stress. The research is a laboratory experimental post-test only with the control group of male Rattus norvegicus exposed to cigarette smoke. The oxidative stress effect in the lung was assessed by MDA levels using ELISA. Differences between groups were analyzed using one-way ANOVA and Tukey’s Post Hoc Test (p < 0.05). The statistical results unveiled that cigarette exposure significantly escalated the levels of MDA compared to the negative control group (p < 0.05). The rat model of cigarette smoke-induced oxidative stress was effective and reproducible, representing a promising and economically viable option for use in studies investigating the pathophysiology of and therapeutic approaches to oxidative stress in the pulmonary.